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dna clean & concentrator-5  (Zymo Research)


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    Structured Review

    Zymo Research dna clean & concentrator-5
    Dna Clean & Concentrator 5, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 8491 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+clean+concentrate+kit/DNA+Clean+%26+Concentrator-5/custom%40d4014%4042484900
    Average 99 stars, based on 8491 article reviews
    dna clean & concentrator-5 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonization
    Article Snippet: Six tubes of 50 μl reactions were prepared for each sample, with the following components: l of Q5 reaction buffer (New England Biolabs: B9027S); 1 μl of dNTP (New England Biolabs: N447L); μl of 10 μM forward primer (barcode_fwd); 2.5 μl of 10 μM reverse primer (barcode_rev); 10 μl of purified DNA; 0.5 μl of Q5 polymerase (New England Biolabs: M0493L); 10 μl of betaine solution (Sigma-Aldrich: B-0300); 13.5 μl of ddH 2 O. .. The PCR conditions were as follows: (1) initial heating at 98 °C for 30 s, (2) 14 cycles of 10 s at 98 °C and 60 s at 72 °C, (3) A final extension at 72 °C for 60 s. The six reactions were pooled into a single sample and purified using the DNA Clean & Concentrate Kit (Zymo Research: D4013), eluted into 20 μl of ddH 2 O, and quantified using a Qubit fluorometer (Thermo Scientific). .. For the second PCR, we added indexes and Illumina P5 and P7 adaptors to the samples.

    Article Title: Direct measurement of sub-kilobase chromatin structure reveals that linker histone H1 broadly compacts chromatin, with differential impact amongst epigenetic states
    Article Snippet: .. The samples were then tagmented with Tn5 at 37°C for 30 minutes, cleaned up with a Zymo DNA Clean & Concentrate kit, and PCR amplified with barcoded primers for an optimized number of cycles determined with a side qPCR reaction. .. Following amplification, libraries were cleaned up, quantified, and sequenced on an Illumina NextSeq 2000 platform to a depth of 30 - 50 million read pairs per technical replicate.

    Article Title: CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonization
    Article Snippet: .. The PCR conditions were as follows: (1) Initial heating at 98 °C for 30 s; (2) 22 cycles of 10 s at 98 °C and 60 s at 72 °C, (3) A final extension at 72 °C for 60 s. The eight reactions were pooled, purified using the DNA Clean & Concentrate Kit (Zymo Research: D4013) and eluted in 20 μl of ddH 2 O. .. The quality of the PCR products was validated using Bioanalyzer High-sensitivity DNA Analysis (Agilent: 5067-4626).

    Article Title: Supporting Information for: Engineering the S-layer of Caulobacter crescentus as a Foundation for Stable, High-Density, 2D Living Materials
    Article Snippet: SpyCatcher was removed from the resulting plasmid to create pBAD-RFP with -SC_RFP_F/R using the Q5 Site-Directed Mutagenesis kit (New England BioLabs) under standard conditions. .. PCR products were visualized on a 1% agarose gels, and purified with QX1 solubilization buffer (Qiagen) and DNA Clean & Concentrate kit (Zymo Research). .. All fragments assembled using HiFi Gibson Assembly 2x Master Mix (New England BioLabs) for 1 hour at 50°C.

    Purification:

    Article Title: CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonization
    Article Snippet: Six tubes of 50 μl reactions were prepared for each sample, with the following components: l of Q5 reaction buffer (New England Biolabs: B9027S); 1 μl of dNTP (New England Biolabs: N447L); μl of 10 μM forward primer (barcode_fwd); 2.5 μl of 10 μM reverse primer (barcode_rev); 10 μl of purified DNA; 0.5 μl of Q5 polymerase (New England Biolabs: M0493L); 10 μl of betaine solution (Sigma-Aldrich: B-0300); 13.5 μl of ddH 2 O. .. The PCR conditions were as follows: (1) initial heating at 98 °C for 30 s, (2) 14 cycles of 10 s at 98 °C and 60 s at 72 °C, (3) A final extension at 72 °C for 60 s. The six reactions were pooled into a single sample and purified using the DNA Clean & Concentrate Kit (Zymo Research: D4013), eluted into 20 μl of ddH 2 O, and quantified using a Qubit fluorometer (Thermo Scientific). .. For the second PCR, we added indexes and Illumina P5 and P7 adaptors to the samples.

    Article Title: FLOWERING LOCUS T genes MtFTb1 and MtFTb2 act redundantly to promote flowering under long days in Medicago truncatula
    Article Snippet: .. Amplification products were analysed by agarose gel electrophoresis, purified using the DNA Clean & Concentrate Kit (Zymo), and sent for sequence analysis (Macrogen, www.macrogen.com ). ..

    Article Title: CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonization.
    Article Snippet: Six tubes of 50 μl reactions were prepared for each sample, with the following components: l of Q5 reaction buffer (New England Biolabs: B9027S); 1 μl of dNTP (New England Biolabs: N447L); μl of 10 μM forward primer (barcode_fwd); 2.5 μl of 10 μM reverse primer (barcode_rev); 10 μl of purified DNA; 0.5 μl of Q5 polymerase (New England Biolabs: M0493L); 10 μl of betaine solution (Sigma-Aldrich: B-0300); 13.5 μl of ddH2O The PCR conditions were as follows: (1) initial heating at 98 ̊C for 30 seconds, (2) 14 cycles of 10 seconds at 98 ̊C and 60 seconds at 72 ̊C, (3) A final extension at 72 C̊ for 60 seconds. .. AR TI CL E IN P RE SS The six reactions were pooled into a single sample and purified using the DNA Clean & Concentrate Kit (Zymo Research: D4013), eluted into 20 μl of ddH2O, and quantified using a Qubit fluorometer (Thermo Scientific). .. Six tubes of 50 μl reactions were prepared for each sample, with the following components: l of Q5 reaction buffer (New England Biolabs: B9027S); 1 μl of dNTP (New England Biolabs: N447L); μl of 10 μM forward primer (barcode_fwd); 2.5 μl of 10 μM reverse primer (barcode_rev); 10 μl of purified DNA; 0.5 μl of Q5 polymerase (New England Biolabs: M0493L); 10 μl of betaine solution (Sigma-Aldrich: B-0300); 13.5 μl of ddH2O The PCR conditions were as follows: (1) initial heating at 98 ̊C for 30 seconds, (2) 14 cycles of 10 seconds at 98 ̊C and 60 seconds at 72 ̊C, (3) A final extension at 72 C̊ for 60 seconds.

    Article Title: CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonization.
    Article Snippet: Selective reverse transcription was carried out using SuperScript II Reverse Transcriptase (invitrogen: 18064022) with a 1st strand synthesis primer (Table S4: cDNA_syn), following the manufacturer's protocol. .. The eight reactions were pooled, purified using the DNA Clean & Concentrate Kit (Zymo Research: D4013) and eluted in 20 μl of ddH2O. .. The quality of the PCR products was validated using Bioanalyzer High-sensitivity DNA Analysis (Agilent: 5067-4626).

    Article Title: CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonization
    Article Snippet: .. The PCR conditions were as follows: (1) Initial heating at 98 °C for 30 s; (2) 22 cycles of 10 s at 98 °C and 60 s at 72 °C, (3) A final extension at 72 °C for 60 s. The eight reactions were pooled, purified using the DNA Clean & Concentrate Kit (Zymo Research: D4013) and eluted in 20 μl of ddH 2 O. .. The quality of the PCR products was validated using Bioanalyzer High-sensitivity DNA Analysis (Agilent: 5067-4626).

    Article Title: Supporting Information for: Engineering the S-layer of Caulobacter crescentus as a Foundation for Stable, High-Density, 2D Living Materials
    Article Snippet: SpyCatcher was removed from the resulting plasmid to create pBAD-RFP with -SC_RFP_F/R using the Q5 Site-Directed Mutagenesis kit (New England BioLabs) under standard conditions. .. PCR products were visualized on a 1% agarose gels, and purified with QX1 solubilization buffer (Qiagen) and DNA Clean & Concentrate kit (Zymo Research). .. All fragments assembled using HiFi Gibson Assembly 2x Master Mix (New England BioLabs) for 1 hour at 50°C.

    Amplification:

    Article Title: Direct measurement of sub-kilobase chromatin structure reveals that linker histone H1 broadly compacts chromatin, with differential impact amongst epigenetic states
    Article Snippet: .. The samples were then tagmented with Tn5 at 37°C for 30 minutes, cleaned up with a Zymo DNA Clean & Concentrate kit, and PCR amplified with barcoded primers for an optimized number of cycles determined with a side qPCR reaction. .. Following amplification, libraries were cleaned up, quantified, and sequenced on an Illumina NextSeq 2000 platform to a depth of 30 - 50 million read pairs per technical replicate.

    Article Title: FLOWERING LOCUS T genes MtFTb1 and MtFTb2 act redundantly to promote flowering under long days in Medicago truncatula
    Article Snippet: .. Amplification products were analysed by agarose gel electrophoresis, purified using the DNA Clean & Concentrate Kit (Zymo), and sent for sequence analysis (Macrogen, www.macrogen.com ). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Direct measurement of sub-kilobase chromatin structure reveals that linker histone H1 broadly compacts chromatin, with differential impact amongst epigenetic states
    Article Snippet: .. The samples were then tagmented with Tn5 at 37°C for 30 minutes, cleaned up with a Zymo DNA Clean & Concentrate kit, and PCR amplified with barcoded primers for an optimized number of cycles determined with a side qPCR reaction. .. Following amplification, libraries were cleaned up, quantified, and sequenced on an Illumina NextSeq 2000 platform to a depth of 30 - 50 million read pairs per technical replicate.

    Agarose Gel Electrophoresis:

    Article Title: FLOWERING LOCUS T genes MtFTb1 and MtFTb2 act redundantly to promote flowering under long days in Medicago truncatula
    Article Snippet: .. Amplification products were analysed by agarose gel electrophoresis, purified using the DNA Clean & Concentrate Kit (Zymo), and sent for sequence analysis (Macrogen, www.macrogen.com ). ..

    Sequencing:

    Article Title: FLOWERING LOCUS T genes MtFTb1 and MtFTb2 act redundantly to promote flowering under long days in Medicago truncatula
    Article Snippet: .. Amplification products were analysed by agarose gel electrophoresis, purified using the DNA Clean & Concentrate Kit (Zymo), and sent for sequence analysis (Macrogen, www.macrogen.com ). ..



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